Audra is co-advised by Prof. Rachel Segalman (ChemE) and Prof. Songi Han. Her research focuses on probing water dynamics on polymeric surfaces using a highly controllable polymer platform. Understanding the effects of polymer structure and chemistry on water dynamics will enable deliberate design of specialty materials for use in membrane applications.
Chung-Ta focuses on how different environmental factors affect transmembrante protein function. He is working on the development of a distance measuring tool between protein helices that will allow him and his team to study how protein functions are modulated through the approach of protein structure dynamics.
The focus of Miranda's PhD study is to develop Dynamic Nuclear Polarization (DNP) enhanced NMR method to study complicated bio-solid systems. The bio-solid system she is probing is tau aggregation pathway, which is associated with Alzheimer's diseases. So far, her approach is to design a DNP spin system to overcome the low sensitivity issue of NMR spectrum of tau aggregates. Miranda's research provides her with the knowledge of the state-of-the-art DNP instrumentation, high field EPR experiments, bio solid-state NMR experiments as well as electron & nuclear spin dynamics.
Yanxian currently focuses on the complex coacervation of intrinsically disordered protein (IDP) and RNA. He applies three different methods to his research including magnetic resonance-based techniques to probe conformation and dynamics of IDP and RNA, polymer and colloid characterization approaches on the properties of complex coacervate, and bioinformatic approaches on predicting complex coacervation.
Kendrick is coadvised by Prof. Michelle O'Malley (ChE) and Prof. Songi Han (DCB). His projects with G protein-coupled receptors give him a strong background in molecular cloning and protein expression using E. coli and S. cerevisiae, membrane protein purification and relevant chromatography techniques (IMAC, affinity, and size exclusion), biophysical techniques such as cw-EPR, DEER, and cryo-EM, as well as basic knowledge into computational techniques such as MATLAB, PyMOL, Mathematica, etc. for data processing. He also has a strong interest in biomedically relevant projects, especially those related to medicine, drug discovery, pharmacology, and neurology in human.
Tarnuma's research focuses on the characterization of supported catalytic materials under the direction of Prof. Susannah Scott and Prof. Songi Han. Her interests lie in using advanced magnetic resonance techniques, including both EPR and NMR, to investigate the structural driving forces and underlying mechanisms that dictate kinetic effects in reactions.
Michael's research focuses on characterizing the sequence-dependent function of intrinsically disordered proteins. In particular, Michael works with disordered regions of the A2a GPCR to understand the relative contribution of disordered regions to A2a's oligomerization properties, and the role of truncation of tau in its toxic aggregation behavior.
Thomas is co-advised by Prof. Rachel Segalman (ChemE) and Prof. Songi Han. The focus of his PhD studies is to develop fundamental understanding of the behavior of water at the surface of polymer membranes. He uses advanced magnetic resonance techniques such as Dynamic Nuclear Polarization to investigate the effect of molecular-scale structure and chemical topology on water dynamics and solute adsorption. This knowledge can then inform the inverse design of energy-efficient membranes for water purification.
- PSBN 4614
Blake is coadvised by Prof. Mark Sherwin (Physics) and Prof. Songi Han (DCB). His focus is to work on developing high field EPR methods and performing high field EPR on systems to learn about the behavior of materials in a high field. By doing this, he helps build a better understanding of the processes that are important for the systems of spins at high field, aiming to develop new methods for studying biological systems. Under Mark Sherwin's guidance, Blake has also helped develop the UCSB electron-free laser as a tool for high field EPR.
Kate focuses on understanding the aggregation of tau, an intrinsically disordered protein (IDP) implicated in neurodegenerative diseases including Alzheimer`s Disease. She is interested in how tau can form extraordinarily stable amyloid fibrils, and aim to understand its aggregation behavior and seeding from a structural point of view. Currently, she is working on (1) seeding tau monomer with tau amyloid fibrils and (2) investigating the amyloid structure and fibril stability. She utilizes a number of biophysical techniques in her projects including ThT fluorescence, EPR, and DEER. With the background of polymer materials and engineering, she can provide new insights into biochemical and biophysical problems.
Asif's main focus is method development and application of solid-state dynamic nuclear polarization (DNP) enhanced NMR to study biosolids, in particular aggregation intermediates of Tau proteins. In addition to this, understanding electron spin dynamics under non-continuous microwave irradiation condition is another major goal in the pursuit of improving DNP efficiency in power limited and faster magic-angle spinning frequency regime.
- PSBN 4623B
Yann's research focuses on the mechanisms of IDP aggregation, with a particular interest in the tau protein, involved in Alzheimer's Disease. His aim is to understand the structural transformations involved in aggregation pathways, and the molecular factors (presence of cofactors and seeds, buffer conditions, etc.) determining the properties of mature aggregates.
- Solid-State NMR
- DNP Development
- Structural and Dynamical Studies of Materials Using Magnetic Resonance
Sheetal's research focuses on the methods, development, and applications of DNP and NMR. His doctoral focus was in solid-state NMR, and now he has been working with pulsed DNP methods to improve the technique he used during his doctoral program. Presently, he is involved primarily in projects related to the instumentation and applications of DNP and NMR.
- PSBN 4623B
Tim's aim is to use recently developed arbitrarily waveform generators to improve the current pulsed EPR experiments such as DEER, DQC, and SIFTER. He also aims to make potentially more effective experiments possible. The primary application of his work is to look at distances across proteins particularly for aggregating systems. He is trying to improve the study of distances on aggregating systems, especially on the model system of tau protein.
- (805) 893-2792
- PSBN 4614
Cocoa has experience in high-field solid state nuclear magnetic resonance spectroscopy with a focus on low-gamma and quadrupolar nuclei and a background in dynamic nuclear polarization at high magnetic fields as well as endohedralmetallofullerenes for materials applications.
Past Members & Group Alumni
Present: Associate Professor, Materials Science and Engineering Department, İzmir Institute of Technology, Turkey.
Present: Senior Online Content Developer, The MathWorks.
Ryan's research focuses on the interface between proteins and the surrounding hydration water in order to better understand how hydration water modifies the protein function. By quantifying the motion of the protein as well as the motion of the water, he aims to address the energetic coupling between a protein and its solvating hydration water.
- (805) 893-2792
- PSBN 4623D
Present: Research Associate, Gritstone Oncology
Tristan's main research goal was to understand the effect of surfactants and hydration on membrane protein functions. He was studying the membrane protein proteorhodopsin (PR) because it is easily characterized by its optical properties and is a good model protein for other membrane proteins. He was aiming to modulate PR by adjusting the external and internal surface hydration while in lipid vesicles using different surface active species.
- (626) 283-0587
- PSBN 4650A
Present: Senior Research Scientist, Colgate-Palmolive
Jessica's research in the Han Lab focused on high field electron paramagnetic resonance for the study of biological systems. This research helped build a better understanding of the processes that are important for the systems of spins at high field, aiming to develop new methods for styding biological systems. Jessica was developing a new tool for measuring distances in membrane proteins that will enhance their study of protein structure and dynamics. Under Mark Sherwin's guidance, she also helped develop the UCSB electron free laser as a tool for high field EPR.
Present: Design Verification Test Engineer III, Karl Storz Imaging
Present: Postdoctoral Researcher, University of Colorado, Boulder (Advisor: Prof. Thomas Perkins)
Neil focused on tau protein which aggregates to form long fibers inside neurons in the brain. He worked on understanding the early structural mechanism of the tau aggregation pathway. Tau aggregation is known to be associated with several neurodegenerative diseases known as Tauopathies which also include Alzheimer’s disease. By using double electron electron resonance (DEER), Neil was able to look at conformational changes both before and after inducing aggregation. Through tracking and characterizing specific conformational intermediates, it could be possible to locate structurally distinct targets for the treatment of tau related diseases.
Present: Project Manager, Infineon Technologies, Germany.
Present: Assistant Professor, Chemistry Department, Syracuse University
Andrew is working on elucidating the pathway of the tau protein’s aggregation into fibers, the cause of neurodegenerative diseases known as Tauopathies, including Alzheimer's disease. He assists with DEER spectroscopy distance measurements, ThT fluorescence, and small peptide production using molecular biology. In addition, he is in charge of creating tau mutants via site-directed mutagenesis, expressing and purifying these mutants, and preparing them for magnetic resonance experiments. One of his interests lies in optimizing spin-labelling efficiency for doubly spin-labelled IDPs, which could cut the time of DEER experiments in half. In doing this, he models the kinetics for, and studies the effects of solvent conditions on, multiple chemical reactions.
Present: Postdoctoral Researcher, National Institutes of Health
Ilia's main project involves technique development for high field EPR and DNP (at 7T). Presently, Ilia and his team are developing new approaches for more efficient DNP using unique capabilities of their spectrometer capable of generating arbitrary shaped pulses at 200 GHz.
The second project Ilia works on is part of large multidisciplinary collaboration dedicated to development of underwater adhesives based on mimicking the adhesives used by mussels in the ocean. They employ a range of techniques such as confocal microscopy, microrheology, EPR and Overhauser DNP to characterize the material and molecular properties of recently discovered adhesive coacervate phases.
- (805) 893-2792
- PSBN 4623D
Present: Founder and CEO of OTOjOY LLC
Present: Senior Research Scientist, Schlumberger-Doll Research
Present: Graduate Student Researcher, NSF Fellow, Stanford University (Advisor: Prof. Rajat Rohatgi)
Alisa’s research focuses on the interface of EPR, NMR, and dynamic nuclear polarization (DNP). She is working on hardware development for static DNP at high magnetic fields as well as doing mechanistic studies to better understand how static DNP is influenced by the electron environment and experimental conditions. These findings can then be used to optimize nuclear signals for materials and biological studies.
- (805) 893-2792
- PSBN 4623C
Present: Assistant Professor, St. Mary's College of California
Alicia’s research focuses on instrument design and pushing the technique of NMR, DNP and EPR, with a specific focus designing an MAS-NMR probe that has DNP and EPR capabilities at temperatures below 90 K. Encompassed in this probe design research are fundamental studies on quantifying absolute enhancement factors from various nitroxide radicals and how the electron spin dynamics affect the DNP mechanism, particularly under magic angle spinning NMR. This work also involves applications to heterogeneous catalytic materials, where surface enhanced NMR through DNP can help differentiate the surface species from the bulk of a material.
Present: Postdoctoral Researcher, Centre de Résonance Magnétique Nucléaire (CMRN), Lyon, France (Advisor: Prof. Anne Lesage)
Present: Principal Scientist, Korimako Chemical Limited, New Zealand
Present: Research Associate - Formulation Development, Regeneron Pharmaceuticals, Inc.
Present: Assistant Professor, Massachusett Institute of Technology
Present: Senior Scientist, Abzena
- Postdoctoral Researcher
- Officer at US Department of State
Present: Foreign Affairs Officer and AAAS Science and Technology Policy Fellow, U.S. Department of State
Nikki was coadvised by Prof. Michelle O'Malley (ChE) and Prof. Songi Han (DCB). She worked on characterizing pharmaceutically relevant membrane protein complexes to link changes in structure and dynamics to function. Namely, she worked with a G protein-coupled receptor called the adenosine A2a receptor to elucidate structural details and functional consequences of homo-dimerization. The A2a receptor regulates cardiac function and several processes within the central nervous system; the outcome of this research will facilitate improved rational drug design to target A2a receptor oligomers in the treatment of disorders such as inflammation, fibrosis, schizophrenia and Parkinson's disease.
Present: Senior Scientist at Pfizer
Alex's research focuses on hydration on a molecular level. The question he is most concerned with is how and why are hydrophilic surfaces hydrated in both biological systems as well as non-biological or inorganic systems? He is working on solving this mystery by performing DNP measurements and surface force measurements. Apart from this, he works on the correlattion of measurements of water diffusivity with surface forces specifically for hydrated hydrophilic surfaces.
Present: Instructor, Elite Educational Institute
Present: NMR R&D Scientist, JEOL USA
Present: Associate Director, R&D, Pacira Pharmaceuticals, Inc.
Till worked on a project on hardware design to run ODNP on fuel cells. He and Prof. Songi Han were attempting to analyze conduction pathways in polyelectrolyte membranes with the help of ODNP. They hoped to better understand the difference between the surface (backbone) diffusion and the bulk diffusion using the ODNP. By changing those diffusion behaviors and understanding how membrane diffusion works, they built up the base to obtain a higher efficiency for diffusion through the membrane.